SNAI1 sirna

SNAI1 siRNA (Human)

Cat. Num. AAA8220157

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNAI1; SNAI1 siRNA (Human); SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna; SNAI1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNAI1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 264

• Applicable Applications for SNAI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNAI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNAI1 sirna

siRNA to inhibit SNAI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976.2
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: 29,083 Da
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family zinc finger 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1
• UniProt Protein Name: Zinc finger protein SNAI1
• Protein Family: Protein snail
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna
• UniProt Entry Name: SNAI1_HUMAN

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.

Protein type: C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; kinase binding

Biological Process: osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell migration; hair follicle morphogenesis; mesoderm formation; positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter; palate development; positive regulation of cell migration