SMOC2 sirna

SMOC2 siRNA (Human)

Cat. Num. AAA829789

• Gene Names: SMOC2; DTDP1; SMAP2; MST117; MSTP117; MSTP140; bA37D8.1; bA270C4A.1; dJ421D16.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMOC2; SMOC2 siRNA (Human); SMAP2; SPARC-related modular calcium-binding protein 2; Secreted modular calcium-binding protein 2; SMOC-2; Smooth muscle-associated protein 2; SMAP-2; SMOC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SMOC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for SMOC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SMOC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMOC2 sirna

siRNA to inhibit SMOC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 262050673
• NCBI GeneID: 64094
• NCBI Accession #: NP_001159884.1
• NCBI GenBank Nucleotide #: NM_001166412.1
• UniProt Accession #: Q9H3U7; Q4G169; Q5TAT7; Q5TAT8; Q86VV9; Q96SF3; Q9H1L3; Q9H1L4; Q9H3U0; Q9H4F7; Q9HCV2; B3KPS7
• Molecular Weight: 50,863 Da
• NCBI Official Full Name: SPARC-related modular calcium-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: SPARC related modular calcium binding 2
• NCBI Official Symbol: SMOC2
• NCBI Official Synonym Symbols: DTDP1; SMAP2; MST117; MSTP117; MSTP140; bA37D8.1; bA270C4A.1; dJ421D16.1
• NCBI Protein Information: SPARC-related modular calcium-binding protein 2
• UniProt Protein Name: SPARC-related modular calcium-binding protein 2
• Protein Family: SPARC-related modular calcium-binding protein
• UniProt Gene Name: SMOC2
• UniProt Synonym Gene Names: SMAP2; SMOC-2; SMAP-2
• UniProt Entry Name: SMOC2_HUMAN

NCBI Description

This gene encodes a member of the SPARC family (secreted protein acidic and rich in cysteine/osteonectin/BM-40), which are highly expressed during embryogenesis and wound healing. The gene product is a matricellular protein which promotes matrix assembly and can stimulate endothelial cell proliferation and migration, as well as angiogenic activity. Associated with pulmonary function, this secretory gene product contains a Kazal domain, two thymoglobulin type-1 domains, and two EF-hand calcium-binding domains. The encoded protein may serve as a target for controlling angiogenesis in tumor growth and myocardial ischemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

SMOC2: Promotes matrix assembly and cell adhesiveness. Can stimulate endothelial cell proliferation, migration, as well as angiogenesis. Defects in SMOC2 are the cause of dentin dysplasia typ1 (DTDP1). A dental defect in which both primary and secondary dentitions are affected. The clinical crowns of both permanent and deciduous teeth are of normal shape, form and color in most cases, although they may be slightly opalescent and blue or brown. Teeth may be very mobile and exfoliate spontaneously because of inadequate root formation. On radiographs, the roots are short and may be more pointed than normal. Pulp chambers are usually absent except for a chevron-shaped remnant in the crown. Root canals are usually absent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Calcium-binding; Secreted

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: basement membrane

Molecular Function: heparin binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; signal transduction

Disease: Dentin Dysplasia, Type I