SLX1B sirna

SLX1B siRNA (Human)

Cat. Num. AAA8238673

• Gene Names: SLX1A; GIYD1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLX1B; SLX1B siRNA (Human); GIYD1; SLX1; GIYD2; Structure-specific endonuclease subunit SLX1; GIY-YIG domain-containing protein 1; SLX1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLX1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 275

• Applicable Applications for SLX1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLX1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLX1B sirna

siRNA to inhibit SLX1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62868208
• NCBI GeneID: 548593
• NCBI Accession #: NP_001014999.1
• NCBI GenBank Nucleotide #: NM_001014999.2
• UniProt Accession #: Q9BQ83; Q6NTG6; B7ZME1
• Molecular Weight: 18,012 Da
• NCBI Official Full Name: structure-specific endonuclease subunit SLX1 isoform 1
• NCBI Official Synonym Full Names: SLX1 homolog A, structure-specific endonuclease subunit
• NCBI Official Symbol: SLX1A
• NCBI Official Synonym Symbols: GIYD1
• NCBI Protein Information: structure-specific endonuclease subunit SLX1
• UniProt Protein Name: Structure-specific endonuclease subunit SLX1
• UniProt Gene Name: SLX1A
• UniProt Entry Name: SLX1_HUMAN

NCBI Description

This gene encodes a protein that is an important regulator of genome stability. The protein represents the catalytic subunit of the SLX1-SLX4 structure-specific endonuclease, which can resolve DNA secondary structures that are formed during repair and recombination processes. Two identical copies of this gene are located on the p arm of chromosome 16 due to a segmental duplication; this record represents the more centromeric copy. Alternative splicing results in multiple transcript variants. Read-through transcription also occurs between this gene and the downstream SULT1A3 (sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3) gene. [provided by RefSeq, Nov 2010]

Uniprot Description

SLX1: catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Forms a heterodimer with SLX4. Found in a segmental duplication on p arm of chromosome 16 giving rise to two identical copies of this gene sharing exons with SULT1A3 and SULT1A4. Two alternatively spliced human isoforms have been described.

Protein type: Deoxyribonuclease; DNA repair, damage; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 16p11.2

Molecular Function: protein binding; 5'-flap endonuclease activity; metal ion binding; crossover junction endodeoxyribonuclease activity

Biological Process: DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination