SLC9B2 sirna

SLC9B2 siRNA (Human)

Cat. Num. AAA8234927

• Gene Names: SLC9B2; NHA2; NHE10; NHEDC2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC9B2; SLC9B2 siRNA (Human); NHA2; NHEDC2; Mitochondrial sodium/hydrogen exchanger 9B2; Mitochondrial Na(+)/H(+) exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; NHE domain-containing protein 2; Sodium/hydrogen exchanger-like domain-containing protein 2; Solute carrier family 9 subfamily B member 2; SLC9B2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC9B2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 417

• Applicable Applications for SLC9B2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC9B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC9B2 sirna

siRNA to inhibit SLC9B2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 663429537
• NCBI GeneID: 133308
• NCBI Accession #: NP_001287685.1
• NCBI GenBank Nucleotide #: NM_001300756.1
• UniProt Accession #: Q86UD5; Q6ZMD8; Q96D95; B5ME52
• Molecular Weight: 48,710 Da
• NCBI Official Full Name: mitochondrial sodium/hydrogen exchanger 9B2 isoform 3
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
• NCBI Official Symbol: SLC9B2
• NCBI Official Synonym Symbols: NHA2; NHE10; NHEDC2
• NCBI Protein Information: mitochondrial sodium/hydrogen exchanger 9B2
• UniProt Protein Name: Mitochondrial sodium/hydrogen exchanger 9B2
• Protein Family: Mitochondrial sodium/hydrogen exchanger
• UniProt Gene Name: SLC9B2
• UniProt Synonym Gene Names: NHA2; NHEDC2; NHE domain-containing protein 2
• UniProt Entry Name: SL9B2_HUMAN

NCBI Description

Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).[supplied by OMIM, Mar 2008]

Uniprot Description

NHEDC2: Electroneutral exchange of protons for Na(+) and Li(+) across the inner mitochondrial membrane. Contributes to the organellar volume homeostasis. Required for osteoclast differentiation and bone resorption activity. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrial inner membrane; integral to membrane

Molecular Function: solute:hydrogen antiporter activity

Biological Process: sodium ion transport; transmembrane transport