SLC6A17 sirna

SLC6A17 siRNA (Human)

Cat. Num. AAA828989

• Gene Names: SLC6A17; NTT4; MRT48
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC6A17; SLC6A17 siRNA (Human); NTT4; Sodium-dependent neutral amino acid transporter SLC6A17; Sodium-dependent neurotransmitter transporter NTT4; Solute carrier family 6 member 17; SLC6A17 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC6A17 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 727

• Applicable Applications for SLC6A17 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC6A17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC6A17 sirna

siRNA to inhibit SLC6A17 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58219014
• NCBI GeneID: 388662
• NCBI Accession #: NP_001010898.1
• NCBI GenBank Nucleotide #: NM_001010898.2
• UniProt Accession #: Q9H1V8; Q5T5Q9; A6NEA8; A8K1R7; B9EIR5
• Molecular Weight: 81,001 Da
• NCBI Official Full Name: sodium-dependent neutral amino acid transporter SLC6A17
• NCBI Official Synonym Full Names: solute carrier family 6 (neutral amino acid transporter), member 17
• NCBI Official Symbol: SLC6A17
• NCBI Official Synonym Symbols: NTT4; MRT48
• NCBI Protein Information: sodium-dependent neutral amino acid transporter SLC6A17
• UniProt Protein Name: Sodium-dependent neutral amino acid transporter SLC6A17
• Protein Family: Sodium-dependent neutral amino acid transporter
• UniProt Gene Name: SLC6A17
• UniProt Synonym Gene Names: NTT4
• UniProt Entry Name: S6A17_HUMAN

NCBI Description

The SLC6 family of proteins, which includes SLC6A17, acts as specific transporters for neurotransmitters, amino acids, and osmolytes like betaine, taurine, and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions (Hoglund et al., 2005 [PubMed 16125675]).[supplied by OMIM, Mar 2008]

Uniprot Description

SLC6A17: Functions as a sodium-dependent vesicular transporter selective for proline, glycine, leucine and alanine. In contrast to other members of this neurotransmitter transporter family, does not appear to be chloride-dependent. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A17 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: synaptic vesicle; synaptic vesicle membrane; integral to plasma membrane; cell junction

Molecular Function: neurotransmitter:sodium symporter activity

Biological Process: glycine transport; alanine transport; leucine transport; neutral amino acid transport; neurotransmitter transport; proline transport; transmembrane transport

Disease: Mental Retardation, Autosomal Recessive 48