SLC39A14 sirna

SLC39A14 siRNA (Human)

Cat. Num. AAA8212278

• Gene Names: SLC39A14; NET34; ZIP14; cig19; LZT-Hs4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC39A14; SLC39A14 siRNA (Human); KIAA0062; ZIP14; Zinc transporter ZIP14; LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; Solute carrier family 39 member 14; Zrt- and Irt-like protein 14; ZIP-14; SLC39A14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC39A14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 492

• Applicable Applications for SLC39A14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC39A14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC39A14 sirna

siRNA to inhibit SLC39A14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190360568
• NCBI GeneID: 23516
• NCBI Accession #: NP_001121903.1
• NCBI GenBank Nucleotide #: NM_001128431.2
• UniProt Accession #: Q15043; Q6ZME8; Q96BB3; A6NH98; B4DIW3; B6EU88; D3DSR4
• Molecular Weight: 54,057 Da
• NCBI Official Full Name: zinc transporter ZIP14 isoform a
• NCBI Official Synonym Full Names: solute carrier family 39 (zinc transporter), member 14
• NCBI Official Symbol: SLC39A14
• NCBI Official Synonym Symbols: NET34; ZIP14; cig19; LZT-Hs4
• NCBI Protein Information: zinc transporter ZIP14
• UniProt Protein Name: Zinc transporter ZIP14
• Protein Family: Zinc transporter
• UniProt Gene Name: SLC39A14
• UniProt Synonym Gene Names: KIAA0062; ZIP14; LZT-Hs4; ZIP-14
• UniProt Entry Name: S39AE_HUMAN

NCBI Description

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003 [PubMed 12659941]).[supplied by OMIM, Mar 2008]

Uniprot Description

SLC39A14: May mediate cellular uptake of nontransferrin-bound iron. Broad-scope metal ion transporter with a preference for zinc uptake. Belongs to the ZIP transporter (TC 2.A.5) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; lamellipodium; endoplasmic reticulum; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: zinc ion transmembrane transporter activity; ferrous iron transmembrane transporter activity

Biological Process: ferrous iron transport; cellular zinc ion homeostasis; transmembrane transport