SLC36A4 sirna

SLC36A4 siRNA (Human)

Cat. Num. AAA8221599

• Gene Names: SLC36A4; PAT4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC36A4; SLC36A4 siRNA (Human); PAT4; Proton-coupled amino acid transporter 4; Proton/amino acid transporter 4; Solute carrier family 36 member 4; SLC36A4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC36A4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 369

• Applicable Applications for SLC36A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC36A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC36A4 sirna

siRNA to inhibit SLC36A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 554790283
• NCBI GeneID: 120103
• NCBI Accession #: NP_001273068.1
• NCBI GenBank Nucleotide #: NM_001286139.1
• UniProt Accession #: Q6YBV0; Q86X30; Q8IVM5; Q8N8S6
• Molecular Weight: 41,385 Da
• NCBI Official Full Name: proton-coupled amino acid transporter 4 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 36 (proton/amino acid symporter), member 4
• NCBI Official Symbol: SLC36A4
• NCBI Official Synonym Symbols: PAT4
• NCBI Protein Information: proton-coupled amino acid transporter 4
• UniProt Protein Name: Proton-coupled amino acid transporter 4
• Protein Family: Proton-coupled amino acid transporter
• UniProt Gene Name: SLC36A4
• UniProt Synonym Gene Names: PAT4; Proton/amino acid transporter 4
• UniProt Entry Name: S36A4_HUMAN

NCBI Description

SLC36A4 belongs to the SLC36 family of amino acid transporters based on sequence similarity with other family members (e.g., SLC36A1; MIM 606561). SLC36 proteins contain about 500 amino acids and have 9 to 11 transmembrane domains. Unlike other SLC36 family members, which are proton-coupled amino acid transporters, SLC36A4 is a high-affinity/low-capacity non-proton-coupled amino acid transporter (Pillai and Meredith, 2011 [PubMed 21097500]).[supplied by OMIM, Feb 2011]

Uniprot Description

SLC36A4: Functions as a sodium-independent electroneutral transporter for tryptophan, proline and alanine. Inhibited by sarcosine. Belongs to the amino acid/polyamine transporter 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: vacuolar membrane; integral to membrane

Molecular Function: symporter activity; amino acid transmembrane transporter activity

Biological Process: proline transport; L-alanine transport; tryptophan transport