SLC35D1 sirna

SLC35D1 siRNA (Human)

Cat. Num. AAA8212205

• Gene Names: SLC35D1; UGTREL7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC35D1; SLC35D1 siRNA (Human); KIAA0260; UGTREL7; UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter; UDP-GlcA/UDP-GalNAc transporter; Solute carrier family 35 member D1; UDP-galactose transporter-related protein 7; UGTrel7; SLC35D1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC35D1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 355

• Applicable Applications for SLC35D1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC35D1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC35D1 sirna

siRNA to inhibit SLC35D1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14028875
• NCBI GeneID: 23169
• NCBI Accession #: NP_055954.1
• NCBI GenBank Nucleotide #: NM_015139.2
• UniProt Accession #: Q9NTN3; Q52LU5; Q92548; A8K185; B7Z3X2
• Molecular Weight: 28,816 Da
• NCBI Official Full Name: UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter
• NCBI Official Synonym Full Names: solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1
• NCBI Official Symbol: SLC35D1
• NCBI Official Synonym Symbols: UGTREL7
• NCBI Protein Information: UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter
• UniProt Protein Name: UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter
• Protein Family: UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter
• UniProt Gene Name: SLC35D1
• UniProt Synonym Gene Names: KIAA0260; UGTREL7; UDP-GlcA/UDP-GalNAc transporter; UGTrel7
• UniProt Entry Name: S35D1_HUMAN

NCBI Description

Glycosylation of cellular glycoconjugates occurs in the endoplasmic reticulum (ER) and Golgi compartment, and requires transport of nucleotide sugars from the cytosol into the lumen of the ER and Golgi by specific transporters. The protein encoded by this gene resides in the ER, and transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm to the ER lumen. It may participate in glucuronidation and/or chondroitin sulfate biosynthesis. Mutations in this gene are associated with Schneckenbecken dysplasia.[provided by RefSeq, Sep 2009]

Uniprot Description

SLC35D1: Transports both UDP-glucuronic acid (UDP-GlcA) and UDP- N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm to into the endoplasmic reticulum lumen. May participate in glucuronidation and/or chondroitin sulfate biosynthesis. Defects in SLC35D1 are a cause of Schneckenbecken dysplasia (SCHBCKD). Schneckenbecken dysplagia is a rare, autosomal recessive, lethal short-limbed skeletal dysplasia with platyspondylia. Belongs to the TPT transporter family. SLC35D subfamily.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: UDP-glucuronic acid transmembrane transporter activity

Biological Process: chondroitin sulfate biosynthetic process; UDP-glucuronic acid transport; xenobiotic metabolic process; embryonic skeletal development; carbohydrate transport; UDP-glucuronate biosynthetic process; transmembrane transport

Disease: Schneckenbecken Dysplasia