Standard Curve (Sample)
Bovine F-box only protein 3 (FBXO3) ELISA Kit | FBXO3 elisa kit
Bovine F-box only protein 3 (FBXO3) ELISA Kit
Gene Names
FBXO3; FBA; FBX3
Reactivity
Bovine
Synonyms
F-box only protein 3 (FBXO3); N/A; Bovine F-box only protein 3 (FBXO3) ELISA Kit; FBXO3 elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of FBXO3. No significant cross-reactivity or interference between FBXO3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBXO3 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FBXO3 elisa kit
Intended Uses: This FBXO3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FBXO3. This ELISA kit for research use only!
Principle of the Assay: FBXO3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FBXO3 antibody and an FBXO3-HRP conjugate. The assay sample and buffer are incubated together with FBXO3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO3 concentration since FBXO3 from samples and FBXO3-HRP conjugate compete for the anti-FBXO3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO3 from the sample, fewer sites are left to bind FBXO3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FBXO3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FBXO3 antibody and an FBXO3-HRP conjugate. The assay sample and buffer are incubated together with FBXO3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO3 concentration since FBXO3 from samples and FBXO3-HRP conjugate compete for the anti-FBXO3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO3 from the sample, fewer sites are left to bind FBXO3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FBXO3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,975 Da
NCBI Official Full Name
F-box only protein 3 isoform 2
NCBI Official Synonym Full Names
F-box protein 3
NCBI Official Symbol
FBXO3
NCBI Official Synonym Symbols
FBA; FBX3
NCBI Protein Information
F-box only protein 3; F-box protein FBX3
UniProt Protein Name
F-box only protein 3
UniProt Gene Name
FBXO3
UniProt Synonym Gene Names
FBX3
UniProt Entry Name
FBX3_HUMAN
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Product Notes
The Bovine FBXO3 fbxo3 (Catalog #AAA244611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244611 ELISA Kit recognizes Bovine FBXO3. It is sometimes possible for the material contained within the vial of "F-box only protein 3 (FBXO3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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