SLC25A3 sirna

SLC25A3 siRNA (Human)

Cat. Num. AAA8221863

• Gene Names: SLC25A3; PHC; PTP; OK/SW-cl.48
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC25A3; SLC25A3 siRNA (Human); PHC; Phosphate carrier protein mitochondrial; Phosphate transport protein; PTP; Solute carrier family 25 member 3; SLC25A3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC25A3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 361

• Applicable Applications for SLC25A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC25A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC25A3 sirna

siRNA to inhibit SLC25A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505775
• NCBI GeneID: 5250
• NCBI Accession #: NP_002626.1
• NCBI GenBank Nucleotide #: NM_002635.3
• UniProt Accession #: Q00325; Q7Z7N7; Q96A03; B3KS34
• Molecular Weight: 39,959 Da
• NCBI Official Full Name: phosphate carrier protein, mitochondrial isoform b
• NCBI Official Synonym Full Names: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
• NCBI Official Symbol: SLC25A3
• NCBI Official Synonym Symbols: PHC; PTP; OK/SW-cl.48
• NCBI Protein Information: phosphate carrier protein, mitochondrial
• UniProt Protein Name: Phosphate carrier protein, mitochondrial
• UniProt Gene Name: SLC25A3
• UniProt Synonym Gene Names: PHC; PTP
• UniProt Entry Name: MPCP_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the transport of phosphate into the mitochondrial matrix, either by proton cotransport or in exchange for hydroxyl ions. The protein contains three related segments arranged in tandem which are related to those found in other characterized members of the mitochondrial carrier family. Both the N-terminal and C-terminal regions of this protein protrude toward the cytosol. Multiple alternatively spliced transcript variants have been isolated. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC25A3: Transport of phosphate groups from the cytosol to the mitochondrial matrix. Phosphate is cotransported with H(+). Defects in SLC25A3 are a cause of mitochondrial phosphate carrier deficiency (MPCD). MPCD is a fatal disorder of oxidative phosphorylation. Patients have lactic acidosis, hypertrophic cardiomyopathy and muscular hypotonia and die within the first year of life. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Mitochondrial; Transporter, SLC family

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: mitochondrion; membrane; integral to plasma membrane; mitochondrial inner membrane

Molecular Function: phosphate carrier activity; symporter activity; protein complex binding

Biological Process: generation of precursor metabolites and energy; transport

Disease: Mitochondrial Phosphate Carrier Deficiency