SLC20A1 sirna

SLC20A1 siRNA (Human)

Cat. Num. AAA8219827

• Gene Names: SLC20A1; PIT1; GLVR1; PiT-1; Glvr-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC20A1; SLC20A1 siRNA (Human); GLVR1; PIT1; Sodium-dependent phosphate transporter 1; Gibbon ape leukemia virus receptor 1; GLVR-1; Leukemia virus receptor 1 homolog; Phosphate transporter 1; PiT-1; Solute carrier family 20 member 1; SLC20A1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC20A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 679

• Applicable Applications for SLC20A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC20A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC20A1 sirna

siRNA to inhibit SLC20A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543630
• NCBI GeneID: 6574
• NCBI Accession #: NP_005406.3
• NCBI GenBank Nucleotide #: NM_005415.4
• UniProt Accession #: Q8WUM9; Q08344; Q6DHX8; Q9UQ82
• Molecular Weight: 73,700 Da
• NCBI Official Full Name: sodium-dependent phosphate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 20 (phosphate transporter), member 1
• NCBI Official Symbol: SLC20A1
• NCBI Official Synonym Symbols: PIT1; GLVR1; PiT-1; Glvr-1
• NCBI Protein Information: sodium-dependent phosphate transporter 1
• UniProt Protein Name: Sodium-dependent phosphate transporter 1
• Protein Family: Sodium-dependent phosphate transporter
• UniProt Gene Name: SLC20A1
• UniProt Synonym Gene Names: GLVR1; PIT1; GLVR-1; PiT-1
• UniProt Entry Name: S20A1_HUMAN

NCBI Description

The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism, signal transduction, and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.[provided by RefSeq, Mar 2011]

Uniprot Description

SLC20A1: Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV). Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: signal transducer activity; high affinity inorganic phosphate:sodium symporter activity; receptor activity; sodium:phosphate symporter activity; inorganic phosphate transmembrane transporter activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; transport; ion transport; signal transduction; phosphate metabolic process; transmembrane transport