SH3D19 sirna

SH3D19 siRNA (Human)

Cat. Num. AAA8227205

• Gene Names: SH3D19; EBP; EVE1; Kryn; SH3P19
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH3D19; SH3D19 siRNA (Human); SH3 domain-containing protein 19; ADAM-binding protein Eve-1; EEN-binding protein; EBP; SH3D19 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SH3D19 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 397

• Applicable Applications for SH3D19 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH3D19 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH3D19 sirna

siRNA to inhibit SH3D19 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 193083141
• NCBI GeneID: 152503
• NCBI Accession #: NP_001009555.3
• NCBI GenBank Nucleotide #: NM_001009555.3
• UniProt Accession #: Q5HYK7; Q08EK1; Q32N10; Q5U3B8; Q86XB3; Q8N5E7; Q9UFC8; B7Z296
• Molecular Weight: 44,667 Da
• NCBI Official Full Name: SH3 domain-containing protein 19 isoform 1
• NCBI Official Synonym Full Names: SH3 domain containing 19
• NCBI Official Symbol: SH3D19
• NCBI Official Synonym Symbols: EBP; EVE1; Kryn; SH3P19
• NCBI Protein Information: SH3 domain-containing protein 19
• UniProt Protein Name: SH3 domain-containing protein 19
• Protein Family: SH3 domain-containing protein
• UniProt Gene Name: SH3D19
• UniProt Synonym Gene Names: EBP
• UniProt Entry Name: SH319_HUMAN

NCBI Description

This gene encodes a multiple SH3 domain-containing protein, which interacts with other proteins, such as EBP and members of ADAM family, via the SH3 domains. This protein may be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1 by EBP, and regulation of ADAM proteins in the signaling of EGFR-ligand shedding. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

SH3D19: May play a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. May be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1. Plays a role in the regulation of cell morphology and cytoskeletal organization. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: nucleoplasm; Golgi apparatus; plasma membrane; cytosol

Molecular Function: protein binding

Biological Process: regulation of cell morphogenesis; cytoskeleton organization and biogenesis; post-Golgi vesicle-mediated transport; positive regulation of membrane protein ectodomain proteolysis