SH3BP2 sirna

SH3BP2 siRNA (Human)

Cat. Num. AAA8224904

• Gene Names: SH3BP2; 3BP2; CRBM; CRPM; 3BP-2; RES4-23
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH3BP2; SH3BP2 siRNA (Human); 3BP2; SH3 domain-binding protein 2; 3BP-2; SH3BP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SH3BP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 561

• Applicable Applications for SH3BP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH3BP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH3BP2 sirna

siRNA to inhibit SH3BP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170014705
• NCBI GeneID: 6452
• NCBI Accession #: NP_001116153.1
• NCBI GenBank Nucleotide #: NM_001122681.1
• UniProt Accession #: P78314; O00500; O15373; P78315; A6NNC2; B2R5R6; B4DT04; D3DVR0; D6R919
• Molecular Weight: 68,129 Da
• NCBI Official Full Name: SH3 domain-binding protein 2 isoform a
• NCBI Official Synonym Full Names: SH3-domain binding protein 2
• NCBI Official Symbol: SH3BP2
• NCBI Official Synonym Symbols: 3BP2; CRBM; CRPM; 3BP-2; RES4-23
• NCBI Protein Information: SH3 domain-binding protein 2
• UniProt Protein Name: SH3 domain-binding protein 2
• Protein Family: SH3 domain-binding protein
• UniProt Gene Name: SH3BP2
• UniProt Synonym Gene Names: 3BP2; 3BP-2
• UniProt Entry Name: 3BP2_HUMAN

NCBI Description

The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

SH3BP2: an adaptor protein that binds differentially to the SH3 domains of certain proteins. Links the hemopoietic tyrosine kinase Fes to the cytoplasmic membrane in a phosphorylation dependent manner. Regulates FC-epsilon RI -mediated degranulation in rat basophilic leukemia RBL-2H3 cells. Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas. Defects in 3BP2 are the cause of cherubism, an autosomal dominant inherited syndrome. Two differentially spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4p16.3

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding

Biological Process: positive regulation of signal transduction; signal transduction

Disease: Cherubism