SETDB1 sirna

SETDB1 siRNA (Human)

Cat. Num. AAA8202235

• Gene Names: SETDB1; ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SETDB1; SETDB1 siRNA (Human); KIAA0067; KMT1E; Histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SETDB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1291

• Applicable Applications for SETDB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SETDB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SETDB1 sirna

siRNA to inhibit SETDB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224177467
• NCBI GeneID: 9869
• NCBI Accession #: NP_001138887.1
• NCBI GenBank Nucleotide #: NM_001145415.1
• UniProt Accession #: Q15047; Q5SZD8; Q5SZD9; Q5SZE0; Q5SZE7; Q96GM9; A6NEW2
• Molecular Weight: 143,001 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETDB1 isoform 1
• NCBI Official Synonym Full Names: SET domain, bifurcated 1
• NCBI Official Symbol: SETDB1
• NCBI Official Synonym Symbols: ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• NCBI Protein Information: histone-lysine N-methyltransferase SETDB1
• UniProt Protein Name: Histone-lysine N-methyltransferase SETDB1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETDB1
• UniProt Synonym Gene Names: KIAA0067; KMT1E; ESET; H3-K9-HMTase 4
• UniProt Entry Name: SETB1_HUMAN

NCBI Description

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]

Uniprot Description

SETDB1: a protein lysine methyltransferase that specifically trimethylates K9 of histone H3 (H3K9me3), a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Unlike SUV39H H3K9 methyltransferase, which functions mainly in heterochromatin regions such as pericentric heterochromatin, SETDB1 functions mainly in euchromatic regions, playing a central role in the silencing of euchromatic genes. H3K9me3 is coordinated with DNA methylation. Interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. Contains Tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. Is targeted to histone H3 by TIF1B, a factor recruited by KRAB zinc-finger proteins. Recruited by DNMT3A to silenced promoters in cancer cells. May play a role in the pathogenesis of Huntington's disease, since levels of SETDB1 and H3K9me3 are both increased in diseased brains. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation