SART3 sirna

SART3 siRNA (Human)

Cat. Num. AAA8236536

• Gene Names: SART3; P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SART3; SART3 siRNA (Human); KIAA0156; TIP110; Squamous cell carcinoma antigen recognized by T-cells 3; SART-3; hSART-3; Tat-interacting protein of 110 kDa; Tip110; SART3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SART3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 927

• Applicable Applications for SART3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SART3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SART3 sirna

siRNA to inhibit SART3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7661952
• NCBI GeneID: 9733
• NCBI Accession #: NP_055521.1
• NCBI GenBank Nucleotide #: NM_014706.3
• UniProt Accession #: Q15020; Q2M2H0; Q58F06; Q8IUS1; Q96J95; A8K2E4; B7ZKM0
• Molecular Weight: 105,584 Da
• NCBI Official Full Name: squamous cell carcinoma antigen recognized by T-cells 3
• NCBI Official Synonym Full Names: squamous cell carcinoma antigen recognized by T cells 3
• NCBI Official Symbol: SART3
• NCBI Official Synonym Symbols: P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
• NCBI Protein Information: squamous cell carcinoma antigen recognized by T-cells 3
• UniProt Protein Name: Squamous cell carcinoma antigen recognized by T-cells 3
• Protein Family: Squamous cell carcinoma antigen
• UniProt Gene Name: SART3
• UniProt Synonym Gene Names: Tip110
• UniProt Entry Name: SART3_HUMAN

NCBI Description

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

SART3: a ubiquitously expressed protein in proliferating cells. A component of the 7SK snRNP complex composed of P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Transiently associates with the U6 and U4/U6 snRNPs during the spliceosome cycle, helping to regenerate functional U4/U6 snRNPs complexes. Expressed in the nucleus of all of malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas, adenocarcinomas, melanomas and leukemias. However, this protein is undetectable in the nucleus of nonmalignant cell lines or normal tissues, except for the testis. Expressed in the cytoplasm of all proliferating cells, including normal and malignant cells, but not in most cells of normal tissues, except for the testis and the fetal liver. Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; RNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; Cajal body; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus

Molecular Function: U4 snRNA binding; protein binding; U6atac snRNA binding; histone binding; nucleotide binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; assembly of spliceosomal tri-snRNP; nucleosome assembly; spliceosomal snRNP biogenesis; regulation of gene expression; homeostasis of number of cells; cell morphogenesis

Disease: Porokeratosis 3, Disseminated Superficial Actinic Type