RUSC1 sirna

RUSC1 siRNA (Human)

Cat. Num. AAA8220810

• Gene Names: RUSC1; NESCA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RUSC1; RUSC1 siRNA (Human); NESCA; RUN and SH3 domain-containing protein 1; New molecule containing SH3 at the carboxy-terminus; Nesca; RUSC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RUSC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 902

• Applicable Applications for RUSC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RUSC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RUSC1 sirna

siRNA to inhibit RUSC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157412245
• NCBI GeneID: 23623
• NCBI Accession #: NP_001098673.1
• NCBI GenBank Nucleotide #: NM_001105203.1
• UniProt Accession #: Q9BVN2; Q5T9U9; Q5T9V0; Q5T9V1; Q5T9V2; Q9UPY4; Q9Y4T5; B3KWM9
• Molecular Weight: 85,216 Da
• NCBI Official Full Name: RUN and SH3 domain-containing protein 1 isoform a
• NCBI Official Synonym Full Names: RUN and SH3 domain containing 1
• NCBI Official Symbol: RUSC1
• NCBI Official Synonym Symbols: NESCA
• NCBI Protein Information: RUN and SH3 domain-containing protein 1
• UniProt Protein Name: RUN and SH3 domain-containing protein 1
• Protein Family: RUN and SH3 domain-containing protein
• UniProt Gene Name: RUSC1
• UniProt Synonym Gene Names: NESCA; Nesca
• UniProt Entry Name: RUSC1_HUMAN

Uniprot Description

RUSC1: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF- dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: microtubule cytoskeleton; Golgi apparatus; postsynaptic membrane; microtubule; cytoplasm; early endosome; postsynaptic density; cytoplasmic membrane-bound vesicle; cytoplasmic vesicle; cell junction; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; actin binding

Biological Process: protein polyubiquitination; positive regulation of signal transduction