RPTOR sirna

RPTOR siRNA (Human)

Cat. Num. AAA8213835

• Gene Names: RPTOR; KOG1; Mip1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPTOR; RPTOR siRNA (Human); KIAA1303; RAPTOR; Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin; TOR)-scaffold protein; RPTOR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RPTOR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1177

• Applicable Applications for RPTOR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPTOR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPTOR sirna

siRNA to inhibit RPTOR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 251823858
• NCBI GeneID: 57521
• NCBI Accession #: NP_001156506.1
• NCBI GenBank Nucleotide #: NM_001163034.1
• UniProt Accession #: Q8N122; Q8N4V9; Q8TB32; Q9P2P3; B2RN36; C6KEF2; F5H7J5
• Molecular Weight: 131,515 Da
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 2
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: RPTOR
• NCBI Official Synonym Symbols: KOG1; Mip1
• NCBI Protein Information: regulatory-associated protein of mTOR
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: RPTOR
• UniProt Synonym Gene Names: KIAA1303; RAPTOR; Raptor
• UniProt Entry Name: RPTOR_HUMAN

NCBI Description

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; cell soma; intracellular membrane-bound organelle; lysosome; lysosomal membrane; cytoplasm; dendrite; cytosol

Molecular Function: protein binding; protein complex binding; protein kinase binding

Biological Process: cellular response to nutrient levels; TOR signaling pathway; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; cell cycle arrest; cell growth; regulation of cell size; positive regulation of TOR signaling pathway