RNF40 sirna

RNF40 siRNA (Human)

Cat. Num. AAA8206753

• Gene Names: RNF40; BRE1B; RBP95; STARING
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF40; RNF40 siRNA (Human); BRE1B; KIAA0661; E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40; RNF40 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF40 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 901

• Applicable Applications for RNF40 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF40 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF40 sirna

siRNA to inhibit RNF40 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 333440440
• NCBI GeneID: 9810
• NCBI Accession #: NP_001193962.1
• NCBI GenBank Nucleotide #: NM_001207033.1
• UniProt Accession #: O75150; Q6AHZ6; Q6N005; Q7L3T6; Q8N615; Q96T18; Q9BSV9; Q9HC82
• Molecular Weight: 102,046 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase BRE1B isoform 2
• NCBI Official Synonym Full Names: ring finger protein 40, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF40
• NCBI Official Synonym Symbols: BRE1B; RBP95; STARING
• NCBI Protein Information: E3 ubiquitin-protein ligase BRE1B
• UniProt Protein Name: E3 ubiquitin-protein ligase BRE1B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF40
• UniProt Synonym Gene Names: BRE1B; KIAA0661; BRE1-B; RBP95
• UniProt Entry Name: BRE1B_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

RNF40: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 16p11.2-p11.1

Cellular Component: nucleoplasm; neuron projection; membrane; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; mRNA 3'-UTR binding; zinc ion binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; protein complex binding; syntaxin-1 binding; ligase activity

Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; regulation of mitotic cell cycle; histone H2B ubiquitination