RNF167 sirna

RNF167 siRNA (Human)

Cat. Num. AAA8206106

• Gene Names: RNF167; LP2254; RING105; 5730408C10Rik
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF167; RNF167 siRNA (Human); E3 ubiquitin-protein ligase RNF167; RING finger protein 167; RING105; RNF167 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF167 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 350

• Applicable Applications for RNF167 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF167 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF167 sirna

siRNA to inhibit RNF167 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14149702
• NCBI GeneID: 26001
• NCBI Accession #: NP_056343.1
• NCBI GenBank Nucleotide #: NM_015528.1
• UniProt Accession #: Q9H6Y7; Q6XYE0; Q8NDC1; Q9Y3V1; D3DTK8
• Molecular Weight: 38,299 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF167
• NCBI Official Synonym Full Names: ring finger protein 167
• NCBI Official Symbol: RNF167
• NCBI Official Synonym Symbols: LP2254; RING105; 5730408C10Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF167
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF167
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF167
• UniProt Entry Name: RN167_HUMAN

NCBI Description

RNF167 is an E3 ubiquitin ligase that interacts with TSSC5 (SLC22A18; MIM 602631) and, together with UBCH6 (UBE2E1; MIM 602916), facilitates TSSC5 polyubiquitylation (Yamada and Gorbsky, 2006 [PubMed 16314844]).[supplied by OMIM, Mar 2008]

Uniprot Description

RNF167: May act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2E1, and then transfers it to substrates, such as SLC22A18. May play a role in growth regulation involved in G1/S transition.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Membrane protein, integral; Ligase

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm; integral to membrane; endomembrane system

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein polyubiquitination; negative regulation of cell cycle