RERG sirna

RERG siRNA (Human)

Cat. Num. AAA8212374

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RERG; RERG siRNA (Human); Ras-related and estrogen-regulated growth inhibitor; RERG sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RERG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 180

• Applicable Applications for RERG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RERG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RERG sirna

siRNA to inhibit RERG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 299758439
• NCBI GeneID: 85004
• NCBI Accession #: NP_001177655.1
• NCBI GenBank Nucleotide #: NM_001190726.1
• UniProt Accession #: Q96A58; B2R9R0; B4DI02
• Molecular Weight: 20,258 Da
• NCBI Official Full Name: ras-related and estrogen-regulated growth inhibitor isoform 2
• NCBI Official Synonym Full Names: RAS-like, estrogen-regulated, growth inhibitor
• NCBI Official Symbol: RERG
• NCBI Protein Information: ras-related and estrogen-regulated growth inhibitor
• UniProt Protein Name: Ras-related and estrogen-regulated growth inhibitor
• Protein Family: Ras-related and estrogen-regulated growth inhibitor
• UniProt Gene Name: RERG
• UniProt Entry Name: RERG_HUMAN

NCBI Description

RERG, a member of the RAS superfamily of GTPases, inhibits cell proliferation and tumor formation (Finlin et al., 2001 [PubMed 11533059]).[supplied by OMIM, Mar 2009]

Uniprot Description

RERG: Binds GDP/GTP and possesses intrinsic GTPase activity. Has higher affinity for GDP than for GTP. In cell lines overexpression leads to a reduction in the rate of proliferation, colony formation and in tumorigenic potential. Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric, Ras; G protein, monomeric

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: membrane; nucleus; cytosol

Molecular Function: GTPase activity; GDP binding; GTP binding; estrogen receptor binding

Biological Process: negative regulation of cell proliferation; metabolic process; small GTPase mediated signal transduction; response to hormone stimulus; negative regulation of cell growth