RASGRP2 sirna

RASGRP2 siRNA (Human)

Cat. Num. AAA8205840

• Gene Names: RASGRP2; CDC25L; CALDAG-GEFI
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RASGRP2; RASGRP2 siRNA (Human); CDC25L; MCG7; RAS guanyl-releasing protein 2; Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B3.3 kinase-like protein; RASGRP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RASGRP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 609

• Applicable Applications for RASGRP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RASGRP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RASGRP2 sirna

siRNA to inhibit RASGRP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 149158727
• NCBI GeneID: 10235
• NCBI Accession #: NP_001092140.1
• NCBI GenBank Nucleotide #: NM_001098670.1
• UniProt Accession #: Q7LDG7; O00538; Q9UL65; A6NDC7
• Molecular Weight: 69,320 Da
• NCBI Official Full Name: RAS guanyl-releasing protein 2
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• NCBI Official Symbol: RASGRP2
• NCBI Official Synonym Symbols: CDC25L; CALDAG-GEFI
• NCBI Protein Information: RAS guanyl-releasing protein 2
• UniProt Protein Name: RAS guanyl-releasing protein 2
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP2
• UniProt Synonym Gene Names: CDC25L; MCG7; CalDAG-GEFI; hCDC25L
• UniProt Entry Name: GRP2_HUMAN

NCBI Description

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RASGRP2 iso2: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. Belongs to the RASGRP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: neuron projection; plasma membrane; synapse; cell junction; cytosol

Molecular Function: diacylglycerol binding; guanyl-nucleotide exchange factor activity; calcium ion binding; lipid binding

Biological Process: platelet activation; Ras protein signal transduction; regulation of cell growth; signal transduction; blood coagulation

Disease: Bleeding Disorder, Platelet-type, 18