RASGRF2 sirna

RASGRF2 siRNA (Human)

Cat. Num. AAA8207491

• Gene Names: RASGRF2; GRF2; RAS-GRF2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RASGRF2; RASGRF2 siRNA (Human); GRF2; Ras-specific guanine nucleotide-releasing factor 2; Ras-GRF2; Ras guanine nucleotide exchange factor 2; RASGRF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RASGRF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,237

• Applicable Applications for RASGRF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RASGRF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RASGRF2 sirna

siRNA to inhibit RASGRF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38505170
• NCBI GeneID: 5924
• NCBI Accession #: NP_008840.1
• NCBI GenBank Nucleotide #: NM_006909.2
• UniProt Accession #: O14827; Q9UK56; B9EG89
• Molecular Weight: 140,764 Da
• NCBI Official Full Name: ras-specific guanine nucleotide-releasing factor 2
• NCBI Official Synonym Full Names: Ras protein-specific guanine nucleotide-releasing factor 2
• NCBI Official Symbol: RASGRF2
• NCBI Official Synonym Symbols: GRF2; RAS-GRF2
• NCBI Protein Information: ras-specific guanine nucleotide-releasing factor 2
• UniProt Protein Name: Ras-specific guanine nucleotide-releasing factor 2
• Protein Family: Ras-specific guanine nucleotide-releasing factor
• UniProt Gene Name: RASGRF2
• UniProt Synonym Gene Names: GRF2; Ras-GRF2
• UniProt Entry Name: RGRF2_HUMAN

NCBI Description

RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011]

Uniprot Description

RASGRF2: Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: endoplasmic reticulum membrane; plasma membrane; cytosol

Molecular Function: calmodulin binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: synaptic transmission; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction