RASGRF1 sirna

RASGRF1 siRNA (Human)

Cat. Num. AAA8236200

• Gene Names: RASGRF1; GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RASGRF1; RASGRF1 siRNA (Human); CDC25; GNRP; GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; Ras-specific nucleotide exchange factor CDC25; RASGRF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RASGRF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,257

• Applicable Applications for RASGRF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RASGRF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RASGRF1 sirna

siRNA to inhibit RASGRF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24797099
• NCBI GeneID: 5923
• NCBI Accession #: NP_002882.3
• NCBI GenBank Nucleotide #: NM_002891.4
• UniProt Accession #: Q13972; Q16027; F8VPA5; H0YKF2; J3KQP9
• Molecular Weight: 143,218 Da
• NCBI Official Full Name: ras-specific guanine nucleotide-releasing factor 1 isoform 1
• NCBI Official Synonym Full Names: Ras protein-specific guanine nucleotide-releasing factor 1
• NCBI Official Symbol: RASGRF1
• NCBI Official Synonym Symbols: GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
• NCBI Protein Information: ras-specific guanine nucleotide-releasing factor 1
• UniProt Protein Name: Ras-specific guanine nucleotide-releasing factor 1
• Protein Family: Ras-specific guanine nucleotide-releasing factor
• UniProt Gene Name: RASGRF1
• UniProt Synonym Gene Names: CDC25; GNRP; GRF1; Ras-GRF1; GNRP
• UniProt Entry Name: RGRF1_HUMAN

NCBI Description

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Uniprot Description

RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: neuron projection; growth cone; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; glutamate receptor binding; guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: regulation of Ras protein signal transduction; regulation of Rac protein signal transduction; cell proliferation; synaptic transmission; long-term memory; small GTPase mediated signal transduction; regulation of synaptic plasticity; positive regulation of Ras protein signal transduction; signal transduction; neurite development; regulation of neuronal synaptic plasticity