RAP2A sirna

RAP2A siRNA (Human)

Cat. Num. AAA8233338

• Gene Names: RAP2A; KREV; RAP2; K-REV; RbBP-30
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAP2A; RAP2A siRNA (Human); Ras-related protein Rap-2a; RbBP-30; RAP2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAP2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 183

• Applicable Applications for RAP2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAP2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAP2A sirna

siRNA to inhibit RAP2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 10518344
• NCBI GeneID: 5911
• NCBI Accession #: NP_066361.1
• NCBI GenBank Nucleotide #: NM_021033.6
• UniProt Accession #: P10114; Q5JSC1; Q5JSC2; B2RCJ1
• Molecular Weight: 20,615 Da
• NCBI Official Full Name: ras-related protein Rap-2a
• NCBI Official Synonym Full Names: RAP2A, member of RAS oncogene family
• NCBI Official Symbol: RAP2A
• NCBI Official Synonym Symbols: KREV; RAP2; K-REV; RbBP-30
• NCBI Protein Information: ras-related protein Rap-2a
• UniProt Protein Name: Ras-related protein Rap-2a
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP2A
• UniProt Entry Name: RAP2A_HUMAN

Uniprot Description

RAP2A: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: recycling endosome; recycling endosome membrane; plasma membrane; midbody; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: microvillus biogenesis; establishment of protein localization; metabolic process; regulation of dendrite morphogenesis; actin cytoskeleton reorganization; establishment of epithelial cell polarity; positive regulation of protein amino acid phosphorylation; regulation of JNK cascade; negative regulation of cell migration; positive regulation of protein amino acid autophosphorylation; Rap protein signal transduction