RAG1 sirna

RAG1 siRNA (Human)

Cat. Num. AAA8200363

• Gene Names: RAG1; RAG-1; RNF74
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAG1; RAG1 siRNA (Human); RNF74; V(D)J recombination-activating protein 1; RAG-1; RING finger protein 74; RAG1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1043

• Applicable Applications for RAG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAG1 sirna

siRNA to inhibit RAG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557841
• NCBI GeneID: 5896
• NCBI Accession #: NP_000439.1
• NCBI GenBank Nucleotide #: NM_000448.2
• UniProt Accession #: P15918; Q8IY72; Q8NER2; E9PPC4
• Molecular Weight: 106,179 Da
• NCBI Official Full Name: V(D)J recombination-activating protein 1
• NCBI Official Synonym Full Names: recombination activating gene 1
• NCBI Official Symbol: RAG1
• NCBI Official Synonym Symbols: RAG-1; RNF74
• NCBI Protein Information: V(D)J recombination-activating protein 1
• UniProt Protein Name: V(D)J recombination-activating protein 1
• Protein Family: Seed allergenic protein
• UniProt Gene Name: RAG1
• UniProt Synonym Gene Names: RNF74; RAG-1
• UniProt Entry Name: RAG1_HUMAN

NCBI Description

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

RAG1: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'- phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1. Homodimer. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Maturing lymphoid cells. Belongs to the RAG1 family.

Protein type: Ubiquitin conjugating system; DNA-binding; EC 3.1.-.-; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; acid-amino acid ligase activity; DNA binding; histone binding; zinc ion binding; sequence-specific DNA binding; metal ion binding; endonuclease activity; ubiquitin-protein ligase activity

Biological Process: histone monoubiquitination; protein autoubiquitination; adaptive immune response; V(D)J recombination; thymus development; B cell differentiation; pre-B cell allelic exclusion; T cell differentiation in the thymus; T cell homeostasis; regulation of T cell differentiation; immune response; negative regulation of caspase activity; DNA recombination

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-positive; Alpha/beta T-cell Lymphopenia With Gamma/delta T-cell Expansion, Severe Cytomegalovirus Infection, And Autoimmunity; Combined Cellular And Humoral Immune Defects With Granulomas; Omenn Syndrome