PTPRH sirna

PTPRH siRNA (Human)

Cat. Num. AAA8240949

• Gene Names: PTPRH; SAP1; R-PTP-H
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPRH; PTPRH siRNA (Human); SAP1; Receptor-type tyrosine-protein phosphatase H; R-PTP-H; Stomach cancer-associated protein tyrosine phosphatase 1; SAP-1; Transmembrane-type protein-tyrosine phosphatase type H; PTPRH sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PTPRH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 937

• Applicable Applications for PTPRH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PTPRH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPRH sirna

siRNA to inhibit PTPRH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 296452983
• NCBI GeneID: 5794
• UniProt Accession #: Q9HD43; Q15426; Q2NKN9; Q2NKP0; C9JCH2
• Molecular Weight: 103,412 Da
• NCBI Official Full Name: Receptor-type tyrosine-protein phosphatase H
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, H
• NCBI Official Symbol: PTPRH
• NCBI Official Synonym Symbols: SAP1; R-PTP-H
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase H
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase H
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRH
• UniProt Synonym Gene Names: SAP1; R-PTP-H; SAP-1
• UniProt Entry Name: PTPRH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Uniprot Description

PTPRH: May contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion- associated substrates and thus negatively regulating integrin- promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of p130cas, focal adhesion kinase and c-Src. Reduces migratory activity of Jurkat cells. Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity

Biological Process: apoptosis; protein amino acid dephosphorylation