PSMC3 sirna

PSMC3 siRNA (Human)

Cat. Num. AAA8222477

• Gene Names: PSMC3; TBP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PSMC3; PSMC3 siRNA (Human); TBP1; 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; Tat-binding protein 1; TBP-1; PSMC3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PSMC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 439

• Applicable Applications for PSMC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PSMC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PSMC3 sirna

siRNA to inhibit PSMC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21361144
• NCBI GeneID: 5702
• NCBI Accession #: NP_002795.2
• NCBI GenBank Nucleotide #: NM_002804.4
• UniProt Accession #: P17980; Q3B757; Q3B865; Q53HU5; Q6GPG8; Q6IBS1; Q96HD3; B2R8V1
• Molecular Weight: 49,204 Da
• NCBI Official Full Name: 26S protease regulatory subunit 6A
• NCBI Official Synonym Full Names: proteasome 26S subunit, ATPase 3
• NCBI Official Symbol: PSMC3
• NCBI Official Synonym Symbols: TBP1
• NCBI Protein Information: 26S protease regulatory subunit 6A
• UniProt Protein Name: 26S protease regulatory subunit 6A
• Protein Family: 26S protease regulatory
• UniProt Gene Name: PSMC3
• UniProt Synonym Gene Names: TBP1; TBP-1
• UniProt Entry Name: PRS6A_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT5: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, suppresses Tat-mediated transactivation. Belongs to the AAA ATPase family.

Protein type: Nuclear receptor co-regulator; Proteasome complex; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; proteasome complex; membrane; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription coactivator activity; ATPase activity; transcription corepressor activity; ATP binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; blastocyst development; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle