Rabbit anti-Human RPS20 Polyclonal Antibody | anti-RPS20 antibody

RPS20, NT (RPS20, 40S ribosomal protein S20) (Azide free) (HRP)

Cat. Num. AAA6343802

• Gene Names: RPS20; S20
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS20; Polyclonal Antibody; NT (RPS20; 40S ribosomal protein S20) (Azide free) (HRP); 40S ribosomal protein S20; anti-RPS20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPS20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human RPS20.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPS20 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RPS20 antibody detected the RPS20 protein (arrow).)

Related Product Information for anti-RPS20 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene.

Product Categories/Family for anti-RPS20 antibody

Antibodies; Ribosomal Proteins

References

Zhao, J., et al. BMC Med. Genet. 11, 96 (2010): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Frum, R., et al. J. Proteome Res. 6(4):1410-1417(2007). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). De Bortoli, M., et al. BMC Cancer 6, 223 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506697
• NCBI GeneID: 6224
• NCBI Accession #: NP_001014.1
• NCBI GenBank Nucleotide #: NM_001023.3
• UniProt Accession #: P60866; P17075; Q5M8S9; B2R4F4; B4DW28
• Molecular Weight: 13,373 Da
• NCBI Official Full Name: 40S ribosomal protein S20 isoform 2
• NCBI Official Synonym Full Names: ribosomal protein S20
• NCBI Official Symbol: RPS20
• NCBI Official Synonym Symbols: S20
• NCBI Protein Information: 40S ribosomal protein S20
• UniProt Protein Name: 40S ribosomal protein S20
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS20
• UniProt Entry Name: RS20_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

RPS20: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q12

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination