PSMA4 sirna

PSMA4 siRNA (Human)

Cat. Num. AAA8224436

• Gene Names: PSMA4; HC9; PSC9; HsT17706
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PSMA4; PSMA4 siRNA (Human); HC9; PSC9; Proteasome subunit alpha type-4; Macropain subunit C9; Multicatalytic endopeptidase complex subunit C9; Proteasome component C9; Proteasome subunit L; PSMA4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PSMA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for PSMA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PSMA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PSMA4 sirna

siRNA to inhibit PSMA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156713442
• NCBI GeneID: 5685
• NCBI Accession #: NP_001096137.1
• NCBI GenBank Nucleotide #: NM_001102667.1
• UniProt Accession #: P25789; Q53XP2; Q567Q5; Q8TBD1; D3DW86
• Molecular Weight: 21,365 Da
• NCBI Official Full Name: proteasome subunit alpha type-4 isoform 1
• NCBI Official Synonym Full Names: proteasome subunit alpha 4
• NCBI Official Symbol: PSMA4
• NCBI Official Synonym Symbols: HC9; PSC9; HsT17706
• NCBI Protein Information: proteasome subunit alpha type-4
• UniProt Protein Name: Proteasome subunit alpha type-4
• Protein Family: Proteasome
• UniProt Gene Name: PSMA4
• UniProt Synonym Gene Names: HC9; PSC9
• UniProt Entry Name: PSA4_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA4: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; EC 3.4.25.1; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis