Mouse STIP1 Monoclonal Antibody | anti-STIP1 antibody

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (AP)

Cat. Num. AAA6164952

• Gene Names: STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: STIP1; Monoclonal Antibody; STIP1 (Stress-Induced-Phosphoprotein 1; HOP; IEF-SSP-3521; P60; STI1; STI1L) (AP); Stress-Induced-Phosphoprotein 1; STI1L; anti-STIP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 20
• Specificity: Recognizes STIP1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 543

• Applicable Applications for anti-STIP1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: STIP1 (AAH02987.1, 1aa-543aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)

Flow Cytometry (FC/FACS)

(FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.)

Flow Cytometry (FC/FACS)

(FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)

Flow Cytometry (FC/FACS)

(FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.)

Flow Cytometry (FC/FACS)

(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

Flow Cytometry (FC/FACS)

(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

Related Product Information for anti-STIP1 antibody

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]). [supplied by OMIM]

Product Categories/Family for anti-STIP1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12804257
• NCBI GeneID: 10963
• NCBI Official Full Name: Stress-induced-phosphoprotein 1
• NCBI Official Synonym Full Names: stress induced phosphoprotein 1
• NCBI Official Symbol: STIP1
• NCBI Official Synonym Symbols: HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• NCBI Protein Information: stress-induced-phosphoprotein 1
• Protein Family: STIP1 homology and U box-containing protein

NCBI Description

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]