PRMT8 sirna

PRMT8 siRNA (Human)

Cat. Num. AAA8212754

• Gene Names: PRMT8; HRMT1L3; HRMT1L4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRMT8; PRMT8 siRNA (Human); HRMT1L3; HRMT1L4; Protein arginine N-methyltransferase 8; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4; PRMT8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRMT8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 385

• Applicable Applications for PRMT8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRMT8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRMT8 sirna

siRNA to inhibit PRMT8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 374858040
• NCBI GeneID: 56341
• NCBI Accession #: NP_001243465.1
• NCBI GenBank Nucleotide #: NM_001256536.1
• UniProt Accession #: Q9NR22; Q8TBJ8; B2RDP0
• Molecular Weight: 44,169 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 8 isoform 2
• NCBI Official Synonym Full Names: protein arginine methyltransferase 8
• NCBI Official Symbol: PRMT8
• NCBI Official Synonym Symbols: HRMT1L3; HRMT1L4
• NCBI Protein Information: protein arginine N-methyltransferase 8
• UniProt Protein Name: Protein arginine N-methyltransferase 8
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT8
• UniProt Synonym Gene Names: HRMT1L3; HRMT1L4
• UniProt Entry Name: ANM8_HUMAN

NCBI Description

Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]

Uniprot Description

PRMT8: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS. Belongs to the protein arginine N-methyltransferase family. PRMT8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase, protein arginine; Methyltransferase

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein-arginine omega-N monomethyltransferase activity; identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; S-adenosylmethionine-dependent methyltransferase activity; histone-arginine N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; regulation of transcription, DNA-dependent; regulation of protein binding; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine