PREB sirna

PREB siRNA (Human)

Cat. Num. AAA8203590

• Gene Names: PREB; SEC12
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PREB; PREB siRNA (Human); SEC12; Prolactin regulatory element-binding protein; Mammalian guanine nucleotide exchange factor mSec12; PREB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PREB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 417

• Applicable Applications for PREB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PREB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PREB sirna

siRNA to inhibit PREB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7019503
• NCBI GeneID: 10113
• NCBI Accession #: NP_037520.1
• NCBI GenBank Nucleotide #: NM_013388.4
• UniProt Accession #: Q9HCU5; Q53SZ8; Q9UH94
• Molecular Weight: 45,468 Da
• NCBI Official Full Name: prolactin regulatory element-binding protein
• NCBI Official Synonym Full Names: prolactin regulatory element binding
• NCBI Official Symbol: PREB
• NCBI Official Synonym Symbols: SEC12
• NCBI Protein Information: prolactin regulatory element-binding protein
• UniProt Protein Name: Prolactin regulatory element-binding protein
• Protein Family: Preblooming protein
• UniProt Gene Name: PREB
• UniProt Synonym Gene Names: SEC12
• UniProt Entry Name: PREB_HUMAN

NCBI Description

This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. [provided by RefSeq, Jul 2008]

Uniprot Description

PREB: Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription. Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; membrane; integral to membrane; nucleus

Molecular Function: DNA binding

Biological Process: COPII coating of Golgi vesicle; protein transport; ER to Golgi vesicle-mediated transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; transcription, DNA-dependent; unfolded protein response; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification