PRDX5 sirna

PRDX5 siRNA (Human)

Cat. Num. AAA8219462

• Gene Names: PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDX5; PRDX5 siRNA (Human); ACR1; Peroxiredoxin-5 mitochondrial; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase; PRDX5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDX5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 214

• Applicable Applications for PRDX5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDX5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDX5 sirna

siRNA to inhibit PRDX5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6912238
• NCBI GeneID: 25824
• NCBI Accession #: NP_036226.1
• NCBI GenBank Nucleotide #: NM_012094.4
• UniProt Accession #: P30044; Q14CK0; Q6IAF2; Q9UBU5; Q9UJU4; Q9UKX4; A6NC19; A6NG06; B7ZLJ4; B7ZVW3
• Molecular Weight: 12,897 Da
• NCBI Official Full Name: peroxiredoxin-5, mitochondrial isoform a
• NCBI Official Synonym Full Names: peroxiredoxin 5
• NCBI Official Symbol: PRDX5
• NCBI Official Synonym Symbols: PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
• NCBI Protein Information: peroxiredoxin-5, mitochondrial
• UniProt Protein Name: Peroxiredoxin-5, mitochondrial
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX5
• UniProt Synonym Gene Names: ACR1; AOEB166; Prx-V
• UniProt Entry Name: PRDX5_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase III promoter; negative regulation of oxidoreductase activity; negative regulation of caspase activity; response to oxidative stress; inflammatory response; negative regulation of apoptosis