PPP1R8 sirna

PPP1R8 siRNA (Human)

Cat. Num. AAA8230421

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP1R8; PPP1R8 siRNA (Human); ARD1; NIPP1; Nuclear inhibitor of protein phosphatase 1; NIPP-1; Protein phosphatase 1 regulatory inhibitor subunit 8; PPP1R8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PPP1R8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 127

• Applicable Applications for PPP1R8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPP1R8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP1R8 sirna

siRNA to inhibit PPP1R8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI GeneID: 5511
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NM_002713.3
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 13,344 Da
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.4.-; Protein phosphatase, regulatory subunit; Inhibitor; Ribonuclease

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; ribonuclease E activity; DNA binding; protein phosphatase type 1 regulator activity; RNA binding; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; negative regulation of catalytic activity; RNA catabolic process; mRNA processing