POR sirna

POR siRNA (Human)

Cat. Num. AAA8203947

• Gene Names: POR; CPR; CYPOR; P450R
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POR; POR siRNA (Human); CYPOR; NADPH--cytochrome P450 reductase; CPR; P450R; POR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 680

• Applicable Applications for POR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POR sirna

siRNA to inhibit POR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 127139033
• NCBI GeneID: 5447
• NCBI Accession #: NP_000932.3
• NCBI GenBank Nucleotide #: NM_000941.2
• UniProt Accession #: P16435; Q16455; Q197M5; Q8N181; Q9H3M8; Q9UDT3
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: NADPH--cytochrome P450 reductase
• NCBI Official Synonym Full Names: P450 (cytochrome) oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Porin
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CYPOR; CPR; P450R
• UniProt Entry Name: NCPR_HUMAN

NCBI Description

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: EC 1.6.2.4; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; membrane; intracellular membrane-bound organelle

Molecular Function: protein binding; enzyme binding; FAD binding; electron carrier activity; NADPH-hemoprotein reductase activity; hydrolase activity; FMN binding; cytochrome-b5 reductase activity; nitric oxide dioxygenase activity; iron ion binding; NADP binding; iron-cytochrome-c reductase activity

Biological Process: response to drug; nitric oxide catabolic process; internal peptidyl-lysine acetylation; positive regulation of monooxygenase activity; positive regulation of cholesterol biosynthetic process; negative regulation of caspase activity; negative regulation of lipase activity; nitrate catabolic process; positive regulation of chondrocyte differentiation; flavonoid metabolic process; positive regulation of smoothened signaling pathway; fatty acid oxidation; carnitine metabolic process; response to nutrient

Disease: Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency; Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis