Rabbit anti-Human POR Polyclonal Antibody | anti-POR antibody

Anti-POR Antibody, Rabbit Polyclonal

Cat. Num. AAA8104007

• Gene Names: POR; CPR; CYPOR; P450R
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: POR; Polyclonal Antibody; Anti-POR Antibody; Rabbit Polyclonal; POR/CYPOR Antibody; Rabbit PAb; Antigen Affinity Purified; P450 (cytochrome) oxidoreductase; Anti-CPR Antibody; Anti-CYPOR Antibody; Anti-P450R Antibody; anti-POR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human POR/CYPOR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-POR antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human POR/CYPOR
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human POR/CYPOR, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-POR rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:77 kDa Observed band size:77 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human POR in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human POR in human ovarian cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-POR antibody

POR (Cytochrome P450 Oxidoreductase, also known as CYPOR) is a Protein Coding gene. The POR gene, located on 7q11.23, is conserved in chimpanzee, Rhesus monkey, dog, and cow, etc. CYPOR is a microsomal electron-transferring enzyme containing both FAD and FMN as co-factors, which provides the reducing equivalents to various redox partners, such as cytochromes P450 (CYPs), heme oxygenase (HO), cytochrome b(5), and squalene monooxygenase. Human patients with severe forms of CYPOR mutation show bone defects such as cranio-and humeroradial synostoses and long bone fractures, known as Antley-Bixler-like Syndrome (ABS). CYPOR is essential for electron donation to microsomal cytochrome P450-mediated monooxygenation in such diverse physiological processes as drug metabolism (approximately 85-90% of therapeutic drugs), steroid biosynthesis, and bioactive metabolite production (vitamin D and retinoic acid metabolites).

References

Xia C, et al. (2019) Structural and functional studies of the membrane-binding domain of nadph-cytochrome p450 oxidoreductase. Biochemistry 58 (19): 2408-2418.Xia C, et al. (2011) Structural basis for human nadph-cytochrome p450 oxidoreductase deficiency. Proc Natl Acad Sci U S A 108 (33): 13486-13491.Polusani SR, et al. (2011) Regulation of gap junction function and connexin 43 expression by cytochrome p450 oxidoreductase (cypor). Biochem Biophys Res Commun 411 (3): 490-495.Marohnic CC, et al. (2011) Mutations of human cytochrome p450 reductase differentially modulate heme oxygenase-1 activity and oligomerization. Arch Biochem Biophys 513 (1): 42-50.

NCBI and Uniprot Product Information

• NCBI GI #: 127139033
• NCBI GeneID: 5447
• NCBI Accession #: NP_000932.3
• NCBI GenBank Nucleotide #: NM_000941.2
• UniProt Accession #: P16435; Q16455; Q197M5; Q8N181; Q9H3M8; Q9UDT3
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: NADPH--cytochrome P450 reductase
• NCBI Official Synonym Full Names: cytochrome p450 oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Porin
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CPR; P450R
• UniProt Entry Name: NCPR_HUMAN

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: EC 1.6.2.4; Endoplasmic reticulum; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane

Molecular Function: [methionine synthase] reductase activity; NADPH-hemoprotein reductase activity; protein binding

Biological Process: positive regulation of monooxygenase activity; xenobiotic metabolic process

Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency