POP1 sirna

POP1 siRNA (Human)

Cat. Num. AAA8223506

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POP1; POP1 siRNA (Human); KIAA0061; Ribonucleases P/MRP protein subunit POP1; hPOP1; POP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1024

• Applicable Applications for POP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POP1 sirna

siRNA to inhibit POP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 225007650
• NCBI GeneID: 10940
• NCBI Accession #: NP_001139332.1
• NCBI GenBank Nucleotide #: NM_001145860.1
• UniProt Accession #: Q99575; Q15037; A8K5W9
• Molecular Weight: 114,709 Da
• NCBI Official Full Name: ribonucleases P/MRP protein subunit POP1
• NCBI Official Synonym Full Names: POP1 homolog, ribonuclease P/MRP subunit
• NCBI Official Symbol: POP1
• NCBI Protein Information: ribonucleases P/MRP protein subunit POP1
• UniProt Protein Name: Ribonucleases P/MRP protein subunit POP1
• Protein Family: Ribonucleases P/MRP protein
• UniProt Gene Name: POP1
• UniProt Synonym Gene Names: KIAA0061; hPOP1
• UniProt Entry Name: POP1_HUMAN

NCBI Description

This gene encodes the protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing. This protein is also an autoantigen in patients suffering from connective tissue diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]

Uniprot Description

POP1: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. Defects in POP1 may be the cause of a severe skeletal dysplasia reminiscent of anauxetic dysplasia. Affected individuals show severe growth retardation of prenatal onset, a bone dysplasia affecting the epiphyses and metaphyses of the long bones particularly in the lower limbs, and abnormalities of the spine including irregularly shaped vertebral bodies and marked cervical spine instability.

Protein type: Ribonuclease; Nucleolus; RNA processing; EC 3.1.26.5

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleolar ribonuclease P complex; extracellular space; ribonuclease MRP complex

Molecular Function: protein binding; ribonuclease MRP activity; ribonuclease P activity

Biological Process: RNA processing; tRNA 5'-leader removal; tRNA catabolic process