POMP sirna

POMP siRNA (Human)

Cat. Num. AAA8228789

• Gene Names: POMP; UMP1; HSPC014; C13orf12; PNAS-110
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POMP; POMP siRNA (Human); C13orf12; UMP1; Proteasome maturation protein; Proteassemblin; Protein UMP1 homolog; hUMP1; Voltage-gated K channel beta subunit 4.1; POMP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POMP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 141

• Applicable Applications for POMP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POMP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POMP sirna

siRNA to inhibit POMP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7705429
• NCBI GeneID: 51371
• NCBI Accession #: NP_057016.1
• NCBI GenBank Nucleotide #: NM_015932.5
• UniProt Accession #: Q9Y244; Q9HB69; A5HKJ2; D6MXU3
• Molecular Weight: 15,789 Da
• NCBI Official Full Name: proteasome maturation protein
• NCBI Official Synonym Full Names: proteasome maturation protein
• NCBI Official Symbol: POMP
• NCBI Official Synonym Symbols: UMP1; HSPC014; C13orf12; PNAS-110
• NCBI Protein Information: proteasome maturation protein
• UniProt Protein Name: Proteasome maturation protein
• Protein Family: Proteasome maturation protein
• UniProt Gene Name: POMP
• UniProt Synonym Gene Names: C13orf12; UMP1; hUMP1
• UniProt Entry Name: POMP_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.[provided by RefSeq, Aug 2010]

Uniprot Description

POMP: Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum. Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK). KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer. Belongs to the POMP/UMP1 family.

Protein type: Proteasome complex

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: proteasome assembly

Disease: Keratosis Linearis With Ichthyosis Congenita And Sclerosing Keratoderma