POLR3A sirna

POLR3A siRNA (Human)

Cat. Num. AAA8219655

• Gene Names: POLR3A; ADDH; HLD7; RPC1; RPC155; hRPC155
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLR3A; POLR3A siRNA (Human); DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160; POLR3A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POLR3A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1390

• Applicable Applications for POLR3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLR3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLR3A sirna

siRNA to inhibit POLR3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 39725938
• NCBI GeneID: 11128
• NCBI Accession #: NP_008986.2
• NCBI GenBank Nucleotide #: NM_007055.3
• UniProt Accession #: O14802; Q8IW34; Q8TCW5
• Molecular Weight: 155,641 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC1
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• NCBI Official Symbol: POLR3A
• NCBI Official Synonym Symbols: ADDH; HLD7; RPC1; RPC155; hRPC155
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC1
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC1
• UniProt Gene Name: POLR3A
• UniProt Synonym Gene Names: RNA polymerase III subunit C1; RPC155
• UniProt Entry Name: RPC1_HUMAN

NCBI Description

The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]

Uniprot Description

POLR3A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3A are the cause of leukodystrophy hypomyelinating type 7 with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7). An autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.7.6; Transcription initiation complex; Nucleotide Metabolism - purine; Transferase

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; membrane; cytosol

Molecular Function: DNA binding; zinc ion binding; DNA-directed RNA polymerase activity; ribonucleoside binding; chromatin binding

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism