POLR2H sirna

POLR2H siRNA (Human)

Cat. Num. AAA8210854

• Gene Names: POLR2H; RPB8; RPB17; RPABC3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLR2H; POLR2H siRNA (Human); DNA-directed RNA polymerases I. II. and III subunit RPABC3; RNA polymerases I. II. and III subunit ABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I. II. and III 17.1 kDa polypeptide; RPB17; RPB8 homolog; hRPB8; POLR2H sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POLR2H siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 86

• Applicable Applications for POLR2H sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLR2H gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLR2H sirna

siRNA to inhibit POLR2H expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 523425724
• NCBI GeneID: 5437
• NCBI Accession #: NP_001265627.1
• NCBI GenBank Nucleotide #: NM_001278698.1
• UniProt Accession #: P52434; P53802; Q969R0; C9J413; C9JBJ6; C9JCU7; C9JUA8
• Molecular Weight: 9,664 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 1
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide H
• NCBI Official Symbol: POLR2H
• NCBI Official Synonym Symbols: RPB8; RPB17; RPABC3
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC3
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3
• UniProt Gene Name: POLR2H
• UniProt Synonym Gene Names: RNA polymerases I, II, and III subunit ABC3; hRPB8
• UniProt Entry Name: RPAB3_HUMAN

NCBI Description

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

POLR2H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Belongs to the eukaryotic RPB8 RNA polymerase subunit family.

Protein type: RNA processing; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; Nucleolus; Transferase; Transcription initiation complex; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; nucleus; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter