Mouse Interferon alpha-2 (IFNA2) Monoclonal Antibody | anti-IFNA2 antibody

Interferon alpha-2 (IFNA2)

Cat. Num. AAA4380029

• Gene Names: IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
• Reactivity: Human. Others not known.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: Interferon alpha-2 (IFNA2); Monoclonal Antibody; Alpha 2a interferon; IFN alpha; IFN-alpha-2; IFNA; IFNA2; IFNA2B; Interferon alpha 2a; Interferon alpha 2b; Interferon alpha-2; Interferon alpha-A; LeIF2; LeIFA; anti-IFNA2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: N27
• Specificity: Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFNfamily consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 188

• Applicable Applications for anti-IFNA2 antibody: ELISA (EIA), Blocking Activity, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: ELISA (For coating, order Ab without BSA); Blocking Activity (Order Ab without Azide); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml)
• Immunogen: Purified recombinant human IFN-alpha2
• Cellular Localization: Cytoplasmic & Extracellular (Secreted)
• Positive Control: Stimulated human peripheral blood mononuclear cells
• Epitope Information: Epitopes of N27 (MBS4380029) and N39 (MBS439245) MAbs
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified IFNA2 Mouse Monoclonal Antibody (N27). Confirmation of Purity and Integrity of Antibody.)

References

1. Kurane I et al. Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes. J Exp Med 1987, 166(4):999-1010
2. Lepe-Zuniga JL et al. Production of interferon-alpha induced by dsRNA in human peripheral blood mononuclear cell cultures: role of priming by dsRNA-induced interferons-gamma and -beta. J Interferon Res 1989, 9(4):445-456
3. Sen GC et al.The interferon system. A bird's eye view of its biochemistry. J Biol Chem 1992, 267(8):5017-5020
4. Capon DJ et al. Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides. Mol Cell Biol 1985, 5(4):768-779
5. Kontsek P et al. Peptide-mapping of three neutralizing epitopes into predicted biologically active sites of human interferon-alpha 2. Immunol Lett 1993, 35(3):281-284
6. Pestka S et al. Interferons and their actions. Annu Rev Biochem 1987, 56:727-777
7. Kontsek P et al. Mapping of two immunodominant structures on human interferon alpha 2c and their role in binding to cells. Mol Immunol 1991, 28:1289-1297
8. Aman MJ et al. Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes. Blood 1996, 87(11):4731-4736

NCBI and Uniprot Product Information

• NCBI GI #: 11067751
• NCBI GeneID: 3440
• NCBI Accession #: NP_000596.2
• NCBI GenBank Nucleotide #: NM_000605.3
• UniProt Accession #: P01563; P01564; Q14606; Q6DJX8; Q96KI6; H2DF54; H2DF55
• Molecular Weight: 16-27kDa
• NCBI Official Full Name: interferon alpha-2
• NCBI Official Synonym Full Names: interferon alpha 2
• NCBI Official Symbol: IFNA2
• NCBI Official Synonym Symbols: IFNA; INFA2; IFNA2B; IFN-alphaA
• NCBI Protein Information: interferon alpha-2
• UniProt Protein Name: Interferon alpha-2
• UniProt Gene Name: IFNA2
• UniProt Synonym Gene Names: IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; LeIF A

NCBI Description

This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Use of the recombinant form of this protein has been shown to be effective in reducing the symptoms and duration of the common cold. [provided by RefSeq, Jun 2011]

Uniprot Description

Produced by macrophages, IFN-alpha have antiviral activities.