POLR1B sirna

POLR1B siRNA (Human)

Cat. Num. AAA828101

• Gene Names: POLR1B; RPA2; RPA135; Rpo1-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLR1B; POLR1B siRNA (Human); DNA-directed RNA polymerase I subunit RPA2; RNA polymerase I subunit 2; DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135; POLR1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POLR1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 952

• Applicable Applications for POLR1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLR1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLR1B sirna

siRNA to inhibit POLR1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 212286172
• NCBI GeneID: 84172
• NCBI Accession #: NP_001131076.1
• NCBI GenBank Nucleotide #: NM_001137604.2
• UniProt Accession #: Q9H9Y6; Q2TAM4; Q585T5; Q6ZRR2; Q9H9D3; B7Z6Y7; B7Z823; F5GZX4; F8W898
• Molecular Weight: 107,858 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA2 isoform 2
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide B, 128kDa
• NCBI Official Symbol: POLR1B
• NCBI Official Synonym Symbols: RPA2; RPA135; Rpo1-2
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA2
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA2
• UniProt Gene Name: POLR1B
• UniProt Synonym Gene Names: RNA polymerase I subunit 2; RPA135
• UniProt Entry Name: RPA2_HUMAN

NCBI Description

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]

Uniprot Description

POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described.

Protein type: EC 2.7.7.6; Nucleolus; Transferase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; ribonucleoside binding; metal ion binding

Biological Process: negative regulation of gene expression, epigenetic; nucleologenesis; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; embryo implantation; rRNA transcription