PMAIP1 sirna

PMAIP1 siRNA (Human)

Cat. Num. AAA8229553

• Gene Names: PMAIP1; APR; NOXA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PMAIP1; PMAIP1 siRNA (Human); NOXA; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; Immediate-early-response protein APR; Protein Noxa; PMAIP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PMAIP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 54

• Applicable Applications for PMAIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PMAIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PMAIP1 sirna

siRNA to inhibit PMAIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 10863923
• NCBI GeneID: 5366
• NCBI Accession #: NP_066950.1
• NCBI GenBank Nucleotide #: NM_021127.2
• UniProt Accession #: Q13794; Q8N589; B2R4T7
• Molecular Weight: 14,955 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: PMAIP1
• NCBI Official Synonym Symbols: APR; NOXA
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Phorbol-12-myristate-13-acetate-induced protein
• UniProt Gene Name: PMAIP1
• UniProt Synonym Gene Names: NOXA; PMA-induced protein 1
• UniProt Entry Name: APR_HUMAN

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: mitochondrial outer membrane; mitochondrion; nucleus; cytosol

Molecular Function: protein binding

Biological Process: proteasomal protein catabolic process; caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of apoptosis; response to dsRNA; positive regulation of caspase activity; cellular response to glucose starvation; positive regulation of protein oligomerization; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; positive regulation of DNA damage response, signal transduction by p53 class mediator; regulation of mitochondrial membrane permeability; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; response to UV; response to X-ray