PIGO sirna

PIGO siRNA (Human)

Cat. Num. AAA8209759

• Gene Names: PIGO; HPMRS2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGO; PIGO siRNA (Human); GPI ethanolamine phosphate transferase 3; Phosphatidylinositol-glycan biosynthesis class O protein; PIG-O; PIGO sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIGO siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 672

• Applicable Applications for PIGO sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIGO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGO sirna

siRNA to inhibit PIGO expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 319918882
• NCBI GeneID: 84720
• NCBI Accession #: NP_001188413.1
• NCBI GenBank Nucleotide #: NM_001201484.1
• UniProt Accession #: Q8TEQ8; Q6P154; Q6UX80; Q8TDS8; Q96CS9; Q9BVN9; Q9Y4B0; B1AML3
• Molecular Weight: 73,992 Da
• NCBI Official Full Name: GPI ethanolamine phosphate transferase 3 isoform 2
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class O
• NCBI Official Symbol: PIGO
• NCBI Official Synonym Symbols: HPMRS2
• NCBI Protein Information: GPI ethanolamine phosphate transferase 3
• UniProt Protein Name: GPI ethanolamine phosphate transferase 3
• UniProt Gene Name: PIGO
• UniProt Synonym Gene Names: PIG-O
• UniProt Entry Name: PIGO_HUMAN

NCBI Description

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PIGO: Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond. Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transferase; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.-.-.-

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: transferase activity

Biological Process: cellular protein metabolic process; preassembly of GPI anchor in ER membrane; GPI anchor biosynthetic process; C-terminal protein lipidation; post-translational protein modification

Disease: Hyperphosphatasia With Mental Retardation Syndrome 2