PHF8 sirna

PHF8 siRNA (Human)

Cat. Num. AAA8237873

• Gene Names: PHF8; JHDM1F; MRXSSD; ZNF422
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PHF8; PHF8 siRNA (Human); KIAA1111; ZNF422; Histone lysine demethylase PHF8; PHD finger protein 8; PHF8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PHF8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 878

• Applicable Applications for PHF8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PHF8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PHF8 sirna

siRNA to inhibit PHF8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 296531349
• NCBI GeneID: 23133
• NCBI Accession #: NP_001171825.1
• NCBI GenBank Nucleotide #: NM_001184896.1
• UniProt Accession #: Q9UPP1; Q5H9U5; Q5JPR9; Q5JPS0; Q5JPS2; Q5JPS3; Q5VUJ4; Q7Z6D4; Q9HAH2; B3KMV4; B7Z911
• Molecular Weight: 98,290 Da
• NCBI Official Full Name: histone lysine demethylase PHF8 isoform 1
• NCBI Official Synonym Full Names: PHD finger protein 8
• NCBI Official Symbol: PHF8
• NCBI Official Synonym Symbols: JHDM1F; MRXSSD; ZNF422
• NCBI Protein Information: histone lysine demethylase PHF8
• UniProt Protein Name: Histone lysine demethylase PHF8
• Protein Family: Histone lysine demethylase
• UniProt Gene Name: PHF8
• UniProt Synonym Gene Names: KIAA1111; ZNF422
• UniProt Entry Name: PHF8_HUMAN

NCBI Description

The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

PHF8: a PHD finger protein with N(epsilon)-methyl lysine histone demethylase activity. Associated with mental retardation and cleft lip/palate. Its active site is closely related to those of FBXL10 and 11, all of which are selective for di- and mono-methylated lysine. In contrast, the JMJD2 demethylases are selective for tri- and di-methylated lysine.

Protein type: EC 1.14.11.27; Demethylase; Nucleolus

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nucleus

Molecular Function: protein binding; histone demethylase activity; zinc ion binding; iron ion binding; histone demethylase activity (H3-K36 specific); oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; histone demethylase activity (H3-K9 specific); methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; brain development; mitotic cell cycle; G1/S transition of mitotic cell cycle; positive regulation of transcription from RNA polymerase I promoter

Disease: Siderius X-linked Mental Retardation Syndrome