PHF7 sirna

PHF7 siRNA (Human)

Cat. Num. AAA8219858

• Gene Names: PHF7; HSPC045; HSPC226; NYD-SP6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PHF7; PHF7 siRNA (Human); PHD finger protein 7; Testis development protein NYD-SP6; PHF7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PHF7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 342

• Applicable Applications for PHF7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PHF7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PHF7 sirna

siRNA to inhibit PHF7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 506325002
• NCBI GeneID: 51533
• NCBI Accession #: NP_001265150.1
• NCBI GenBank Nucleotide #: NM_001278221.1
• UniProt Accession #: Q9BWX1; K4DI82
• Molecular Weight: 39,259 Da
• NCBI Official Full Name: PHD finger protein 7 isoform 2
• NCBI Official Synonym Full Names: PHD finger protein 7
• NCBI Official Symbol: PHF7
• NCBI Official Synonym Symbols: HSPC045; HSPC226; NYD-SP6
• NCBI Protein Information: PHD finger protein 7
• UniProt Protein Name: PHD finger protein 7
• Protein Family: PHD finger protein
• UniProt Gene Name: PHF7
• UniProt Entry Name: PHF7_HUMAN

NCBI Description

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

PHF7: May play a role in spermatogenesis.

Protein type: Cell development/differentiation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; microtubule organizing center; nucleus

Molecular Function: zinc ion binding