Western Blot (WB)
(Fig.1. Western Blot analysis of 293T (1), AD293 (2), 22RV1 (3), Hela (4), diluted at 1:2000.)
Rabbit c-Fos Polyclonal Antibody | anti-C-FOS antibody
c-Fos Polyclonal Antibody
IF: 1:50-1:200
IHC-P: 1:100-1:300
ELISA: 1:5000
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig.1. Western Blot analysis of 293T (1), AD293 (2), 22RV1 (3), Hela (4), diluted at 1:2000.)
Western Blot (WB)
(Fig.1. Western Blot analysis of 293T (1), AD293 (2), 22RV1 (3), Hela (4), diluted at 1:2000.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of mouse kidney tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of mouse kidney tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of rat lung tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of rat lung tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human appendix tissue. 1, c-Fos Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human appendix tissue. 1, c-Fos Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
NCBI and Uniprot Product Information
NCBI Description
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Uniprot Description
Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; nucleus; cytosol
Molecular Function: protein binding; double-stranded DNA binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; conditioned taste aversion; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; aging; response to corticosterone stimulus; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; cellular response to hormone stimulus; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to cold; response to progesterone stimulus
Research Articles on C-FOS
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Product Notes
The C-FOS fos (Catalog #AAA9700664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Fos Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-Fos can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IF: 1:50-1:200 IHC-P: 1:100-1:300 ELISA: 1:5000. Researchers should empirically determine the suitability of the C-FOS fos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Fos, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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