PGM5 sirna

PGM5 siRNA (Human)

Cat. Num. AAA8222662

• Gene Names: PGM5; PGMRP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PGM5; PGM5 siRNA (Human); PGMRP; Phosphoglucomutase-like protein 5; Aciculin; Phosphoglucomutase-related protein; PGM-RP; PGM5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PGM5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 567

• Applicable Applications for PGM5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PGM5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PGM5 sirna

siRNA to inhibit PGM5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 133922562
• NCBI GeneID: 5239
• NCBI Accession #: NP_068800.2
• NCBI GenBank Nucleotide #: NM_021965.3
• UniProt Accession #: Q15124; Q5VYV3; Q8N527; Q9UDH4; B1AM46; B4DLQ6
• Molecular Weight: 41,790 Da
• NCBI Official Full Name: phosphoglucomutase-like protein 5
• NCBI Official Synonym Full Names: phosphoglucomutase 5
• NCBI Official Symbol: PGM5
• NCBI Official Synonym Symbols: PGMRP
• NCBI Protein Information: phosphoglucomutase-like protein 5
• UniProt Protein Name: Phosphoglucomutase-like protein 5
• Protein Family: Phosphoglucomutase-like protein
• UniProt Gene Name: PGM5
• UniProt Synonym Gene Names: PGMRP; PGM-RP
• UniProt Entry Name: PGM5_HUMAN

NCBI Description

Phosphoglucomutases (EC 5.2.2.2.), such as PGM5, are phosphotransferases involved in interconversion of glucose-1-phosphate and glucose-6-phosphate. PGM activity is essential in formation of carbohydrates from glucose-6-phosphate and in formation of glucose-6-phosphate from galactose and glycogen (Edwards et al., 1995 [PubMed 8586438]).[supplied by OMIM, Mar 2008]

Uniprot Description

PGM5: Component of adherens-type cell-cell and cell-matrix junctions. Lacks phosphoglucomutase activity. Belongs to the phosphohexose mutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q13

Cellular Component: dystrophin-associated glycoprotein complex; internal side of plasma membrane; costamere; focal adhesion; cell-cell adherens junction; stress fiber; spot adherens junction; Z disc; cytosol; sarcolemma

Molecular Function: phosphoglucomutase activity; magnesium ion binding; structural molecule activity

Biological Process: galactose catabolic process; glycogen biosynthetic process; glucose metabolic process; cell adhesion