PGM1 sirna

PGM1 siRNA (Human)

Cat. Num. AAA8227132

• Gene Names: PGM1; CDG1T; GSD14
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PGM1; PGM1 siRNA (Human); Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; PGM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PGM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 365

• Applicable Applications for PGM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PGM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PGM1 sirna

siRNA to inhibit PGM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 290463102
• NCBI GeneID: 5236
• NCBI Accession #: NP_001166289.1
• NCBI GenBank Nucleotide #: NM_001172818.1
• UniProt Accession #: P36871; Q16105; Q16106; Q5BKZ9; Q6NW22; Q86U74; Q96J40; Q9NTY4; B2R5N9; B4DPV0
• Molecular Weight: 40,283 Da
• NCBI Official Full Name: phosphoglucomutase-1 isoform 2
• NCBI Official Synonym Full Names: phosphoglucomutase 1
• NCBI Official Symbol: PGM1
• NCBI Official Synonym Symbols: CDG1T; GSD14
• NCBI Protein Information: phosphoglucomutase-1
• UniProt Protein Name: Phosphoglucomutase-1
• Protein Family: Phosphoglucomutase
• UniProt Gene Name: PGM1
• UniProt Synonym Gene Names: PGM 1
• UniProt Entry Name: PGM1_HUMAN

NCBI Description

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

PGM1: This enzyme participates in both the breakdown and synthesis of glucose. Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14). A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test. Belongs to the phosphohexose mutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase; Carbohydrate Metabolism - galactose; EC 5.4.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytoplasm; cytosol; actin cytoskeleton

Molecular Function: phosphoglucomutase activity; magnesium ion binding

Biological Process: galactose catabolic process; glycogen biosynthetic process; glycogen catabolic process; glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

Disease: Congenital Disorder Of Glycosylation, Type It