PGAP3 sirna

PGAP3 siRNA (Human)

Cat. Num. AAA8227773

• Gene Names: PGAP3; CAB2; PERLD1; PP1498; hCOS16; AGLA546
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PGAP3; PGAP3 siRNA (Human); CAB2; PERLD1; Post-GPI attachment to proteins factor 3; COS16 homolog; hCOS16; Gene coamplified with ERBB2 protein; PER1-like domain-containing protein 1; PGAP3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PGAP3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 299

• Applicable Applications for PGAP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PGAP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PGAP3 sirna

siRNA to inhibit PGAP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 619726944
• NCBI GeneID: 93210
• NCBI Accession #: NP_001278655.1
• NCBI GenBank Nucleotide #: NM_001291726.1
• UniProt Accession #: Q96FM1; Q86Z03; Q8NBJ8; B4DGK7
• Molecular Weight: 33,967 Da
• NCBI Official Full Name: post-GPI attachment to proteins factor 3 isoform 2
• NCBI Official Synonym Full Names: post-GPI attachment to proteins 3
• NCBI Official Symbol: PGAP3
• NCBI Official Synonym Symbols: CAB2; PERLD1; PP1498; hCOS16; AGLA546
• NCBI Protein Information: post-GPI attachment to proteins factor 3
• UniProt Protein Name: Post-GPI attachment to proteins factor 3
• Protein Family: Post-GPI attachment to proteins factor
• UniProt Gene Name: PGAP3
• UniProt Synonym Gene Names: CAB2; PERLD1; hCOS16
• UniProt Entry Name: PGAP3_HUMAN

NCBI Description

This gene encodes a glycosylphosphatidylinositol (GPI)-specific phospholipase that primarily localizes to the Golgi apparatus. This ubiquitously expressed gene is predicted to encode a seven-transmembrane protein that removes unsaturated fatty acids from the sn-2 position of GPI. The remodeling of the constituent fatty acids on GPI is thought to be important for the proper association between GPI-anchored proteins and lipid rafts. The tethering of proteins to plasma membranes via posttranslational GPI-anchoring is thought to play a role in protein sorting and trafficking. Mutations in this gene cause the autosomal recessive neurologic disorder hyperphosphatasia with mental retardation syndrome 4 (HPMRS4). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

PGAP3: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable). Belongs to the PGAP3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; integral to membrane

Molecular Function: hydrolase activity, acting on ester bonds

Biological Process: GPI anchor metabolic process; GPI anchor biosynthetic process

Disease: Hyperphosphatasia With Mental Retardation Syndrome 4